There is no "magic bullet", but the following may help: - Crystal growth at various temperatures
- Additive screen (such as Hampton Research screen) - Detergent screen - Micro-seeding - Looking for another crystal form, with the option of using a combination of micro-seeding with broad screening Good luck Thierry From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Klaus Fütterer Sent: Wednesday, January 22, 2014 10:02 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Intergrown crystals Dear ccp4bb contributors, We are dealing with the problem of a protein (~ 50 kDa) that crystallises readily, but has an annoying habit of forming highly intergrown rods or needles. Even when the crystals look optically homogenous under the microcsope, diffraction is so so (3.5 Å or so on the synchrotron), but patterns reflect several crystal lattices that the processing software cannot resolve properly. We have tried this: - additive screens - switching the His-tag from N- to C-terminus - cutting the tag - thermal stability screens in a variety of buffers - growth in the presence of potential ligands/substrates Any suggestions for tricks that we haven't thought of so far? Thank you. Klaus ======================================================================= Dr. Klaus Fütterer School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham F: +44-(0)-121-414 5925 Edgbaston E: k.futte...@bham.ac.uk<mailto:k.futte...@bham.ac.uk> Birmingham, B15 2TT, UK W: http://tinyurl.com/futterer-lab ======================================================================= Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
