Dear Nazia, Glad you are trying your hand at a membrane protein. You need to have detergents (like ldao, octylpoe ) in your refolding and purifcn buffer. Best wishes Krishnaswamy Mku On 24 Nov 2013 05:32, "CCP4BB automatic digest system" < lists...@jiscmail.ac.uk> wrote:
> There are 6 messages totaling 1209 lines in this issue. > > Topics of the day: > > 1. hello. > 2. 3D stereo notebook (3) > 3. Plate like crystals > 4. last chance to sign up for CCP4 bursaries - early bird registration > to the > CCP4 Study Weekend 2014 Nottingham > > ---------------------------------------------------------------------- > > Date: Sat, 23 Nov 2013 01:59:54 +0000 > From: Rex Palmer <rex.pal...@btinternet.com> > Subject: hello. > > > > http://portal.limon.gen.tr/gm/itfhmazeeuauqaqvqmzusuu.html > > > > > > > > > > > > > > > Rex Palmer > > > > > > > > > > > ------------------------------ > > Date: Sat, 23 Nov 2013 10:33:46 +0800 > From: zhongzhou chen <zhongzhouc...@sohu.com> > Subject: 3D stereo notebook > > Dear all, > > > I am looking into ordering a stereo notebook to view 3D protein structure. > Which notebook can you recommended? I require that the 3D notebook will > be used with Linux and Windows, so support of the correspoding graphics > card with both systems is required. > > In addition, I found that Dell XPS17XPS17D-428 can be used in windows. > However I do not know whether 3D view is OK or not in Linux? Moreover, the > displayer is 17 inch, I think that it is a little larger. > > Any comments or experience are welcome. > > > > > > > Thanks and best wishes, > > > zhongzhou chen > > china agricultural university > > > > > ------------------------------ > > Date: Sat, 23 Nov 2013 12:26:24 +0100 > From: mesters <mest...@biochem.uni-luebeck.de> > Subject: Re: 3D stereo notebook > > Hello, > > Do not get your fingers burned as far as stereo under linux is > concerned, nvidia's drivers do not support this for the gt and gtx cards > (although these cards may have similar architecture at the end of the > day e.g. kepler 107). > > Under linux you do need a quadro card for stereo and I do not know of a > laptop with a 120Hz capable screen, with build-in IR-emitter (as the USB > based 3D nvidia 3D glasses will not work under Linux) and with a quadro > card (no laptop with a 3-pin as far as I know...)...... > > As far as I can overlook the market, all mobile workstations equipped > with a quadro have no 120Hz screen and require you to connect a 120Hz > screen for stereo viewing and if the screen comes with a build-in > IR-emitter and a DisplayPort 1.2 (or you find youself buying an > expensive adaptor), you can operate it under linux as well. > > - J. - > -- > Dr.Jeroen R. Mesters > Deputy and Group Leader > > Institute of Biochemistry, University of Lübeck > Ratzeburger Allee 160, 23538 Lübeck, Germany > > phone: +49-451-5004065 (secretariate 5004061) > fax: +49-451-5004068 > > http://www.biochem.uni-luebeck.de <Http://www.biochem.uni-luebeck.de> > http://www.iobcr.org <Http://www.iobcr.org> > > > -- > If you can look into the seeds of time and tell which grain will grow > and which will not, speak then to me who neither beg nor fear > (Shakespeare's Macbeth, Act I, Scene 3) > -- > Disclaimer > * This message contains confidential information and is intended only > for the individual named. If you are not the named addressee you should > not disseminate, distribute or copy this e-mail. Please notify the > sender immediately by e-mail if you have received this e-mail by mistake > and delete this e-mail from your system. > * E-mail transmission cannot be guaranteed to be secure or error-free as > information could be intercepted, corrupted, lost, destroyed, arrive > late or incomplete, or contain viruses. The sender therefore does not > accept liability for any errors or omissions in the contents of this > message, which arise as a result of e-mail transmission. If verification > is required please request a hard-copy version. Please send us by fax > any message containing deadlines as incoming e-mails are not screened > for response deadlines. > * Employees of the Institute are expressly required not to make > defamatory statements and not to infringe or authorize any infringement > of copyright or any other legal right by email communications. Any such > communication is contrary to Institute policy and outside the scope of > the employment of the individual concerned. The Institute will not > accept any liability in respect of such communication, and the employee > responsible will be personally liable for any damages or other liability > arising. Employees who receive such an email must notify their > supervisor immediately. > -- > > ------------------------------ > > Date: Sat, 23 Nov 2013 23:07:06 +0530 > From: "Nazia Nasir Phd2009,ProteinCrystall.Lab" < > nazia.nasi...@nii.ac.in> > Subject: Plate like crystals > > Hello everyone, > > I am trying to crystallize the truncated version of a memebrane protein (30 > amino acids truncated, including signal sequence a transmembrane helix). > The protein expresses in inclusion bodies and I solubilize it in 8 M urea > and finally purify it in 20mM Tris, 50mM- 300mM NaCl, 5% glycerol, pH 8.5. > I have been able to gow crystals but all of them grow as thin plates. The > best resltion obtained so far is 3.7 A. Moreover, for SAD phasing using the > natural Sulpurs, the Cr beam gives a maxmum resoltion of 7 A. I have > exhausted all screens in my lab and set up expansions of all positive hits, > but all I get are plate like crystals with poor resoltion. > > Can anyone suggest how to improve my crystals? Moreover, is the data I am > getting so far workable for SAD phasing? mY protein is 500 amino acids long > with 11 Methionines, spread out almost through out the protein length. > > Eagerly awaiting some help! > > Thanks ! > > > > > -- > Nazia Nasir > PhD Scholar > Protein Crystallography Lab > National Institute of Immunology > New Delhi > > ------------------------------ > > Date: Sat, 23 Nov 2013 20:15:59 +0000 > From: "Tews I." <ivo.t...@soton.ac.uk> > Subject: last chance to sign up for CCP4 bursaries - early bird > registration to the CCP4 Study Weekend 2014 Nottingham > > The early bird registration for the CCP4 study weekend closes tomorrow, > 24th November. > > After this date the registration fee increases and there will be no > student bursaries available. > > For registration, please follow the link: > https://eventbooking.stfc.ac.uk/login?EVENT=147 > > > We cordially invite you to participate in the 2014 Study Weekend "Two Way > Street - Complementary Methods". More than half of the deposited structures > in the PDB are oligomeric, and about three quarters are annotated with > ligands. This provides a strong motivation for the CCP4 users to understand > what methods can be used to complement structure analysis and to learn how > complementary techniques can be helpful in structure determination. The > programme can be found at > http://www.cse.scitech.ac.uk/events/CCP4_2014/programme.html > > > You will learn how complementary methods help in structure determination > and analysis, leading to a better understanding of the bio-macromolecules > we study: > > * In-solution methods like SAXS or biophysical techniques allow > validating the macromolecular assembly. > * Biophysical techniques are also essential to probe ligands and > co-factors. > * We address how to make ligand complexes with novel methods to mount > and manipulate crystals. > * A session on drug design gives the perspective and discusses > additional techniques such as NMR. > * Simulations and NMR also address the dynamic nature of > macromolecules. > * EM techniques address macromolecular complexes and go full circle > with the X-ray analysis. > > The organisers. > > > Jon Cooper, UCL > > Ivo Tews, University of Southampton > > University of Southampton, Centre for Biological Sciences > Institute for Life Sciences (IfLS) B85, Highfield Campus > Southampton SO17 1BJ > > phone: ++44 (0)23-8059-4415 lab: -4365 fax: -5159 > http://www.soton.ac.uk/sdc > http://www.southampton.ac.uk/biosci/about/staff/it1y09.page? > > ------------------------------ > > Date: Sat, 23 Nov 2013 17:59:35 -0500 > From: Zhijie Li <zhijie...@utoronto.ca> > Subject: Re: 3D stereo notebook > > Hi Zhongzhou, > > For laptops, the passive stereo (Zalman mode) would be the easiest way to > go. All you need to do is to plug that monitor to the VGA port of you > laptop. The halving of the vertical resolution under stereo mode only > affects reading the characters, which can be solved by setting the fonts > bigger. > > I am not sure if LG D-2342 is still in market. But if not they should have > some newer models of the same technology. > > Zhijie > > From: zhongzhou chen > Sent: Friday, November 22, 2013 9:33 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] 3D stereo notebook > > Dear all, > > > I am looking into ordering a stereo notebook to view 3D protein structure. > Which notebook can you recommended? I require that the 3D notebook will be > used with Linux and Windows, so support of the correspoding graphics card > with both systems is required. > > In addition, I found that Dell XPS17XPS17D-428 can be used in windows. > However I do not know whether 3D view is OK or not in Linux? Moreover, the > displayer is 17 inch, I think that it is a little larger. > > Any comments or experience are welcome. > > > > > > > Thanks and best wishes, > > > zhongzhou chen > > china agricultural university > > > ------------------------------ > > End of CCP4BB Digest - 22 Nov 2013 to 23 Nov 2013 (#2013-332) > ************************************************************* >
