Dear All,
I think i have not explained my problem precisely. This
may be weird one but let me elaborate more. I have have a protein
moleculeA, having N-term, and C-term end. Structurally, it is dimer
with anti-parallel arrangement i.e N-terminal of one copyA of molecule
form dimer in such a way that it copyB would be arranged in
antiparallel fashioned (N-term of copyA is besides C-term of CopyB).
So when i am searching for two copy of molecule in phaser it is giving
me two copy of molecule in parallel arrangement. So my question is,
how to tell phaser that after fixing the orientation of first copy, to
change the orientation of 2nd copy with respect to first one so that
their n-teminal and c-terminal lies beside each other. I am looking
for your valuable suggestion.
Thank you
On 21/11/2013, Matthias Zebisch <matth...@strubi.ox.ac.uk> wrote:
> i think you need to explain the problem in greater detail to the community
>
> eg. how do you come to know that the orientation of mol2 is 180° flipped
> - perhaps it is correct as phaser puts it?
>
> No one will be able to help you with so little information
>
> Best wishes,
>
> Matthias
>
> -----------------------------------------
> Dr. Matthias Zebisch
> Division of Structural Biology,
> Wellcome Trust Centre for Human Genetics,
> University of Oxford,
> Roosevelt Drive,
> Oxford OX3 7BN, UK
>
> Phone (+44) 1865 287549;
> Fax (+44) 1865 287547
> Email matth...@strubi.ox.ac.uk
> Website http://www.strubi.ox.ac.uk
> -----------------------------------------
>
> On 11/21/2013 5:38 PM, Appu kumar wrote:
>> Dear All,
>> I seek your valuable suggestion on a MR problem. I am
>> asking phaser to search for two molecules in ASU, first molecules
>> phaser searched right but when placing the second molecule, it
>> orientation has got flipped by 180 deegree. Is there any way to tell
>> phaser to fix the orientation of second copy of molecules. ? Looking
>> forward for your valuable suggestion
>>
>> Thank you very much in advance
>> Appu
>>
>
>
> --
> -----------------------------------------
> Dr. Matthias Zebisch
> Division of Structural Biology,
> Wellcome Trust Centre for Human Genetics,
> University of Oxford,
> Roosevelt Drive,
> Oxford OX3 7BN, UK
>
> Phone (+44) 1865 287549;
> Fax (+44) 1865 287547
> Email matth...@strubi.ox.ac.uk
> Website http://www.strubi.ox.ac.uk
> -----------------------------------------
>
>
