Hi Bernhard,
If your cheap imidazole works fine aside from the absorption problem, there's
always my favorite stone-age detection method: pencil a numbered grid onto a
piece of filter paper, spot the fractions on it, and stain with coomassie.
It'll tell you which fractions to load on a gel, and it goes easy on the budget
as well.
Phoebe
++++++++++++++++++++++++++++++++++++++++++
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
773 834 1723; pr...@uchicago.edu<mailto:pr...@uchicago.edu>
http://bmb.bsd.uchicago.edu/Faculty_and_Research/
http://www.rsc.org/shop/books/2008/9780854042722.asp
________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bernhard Rupp
[hofkristall...@gmail.com]
Sent: Wednesday, August 21, 2013 9:33 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Low 280 absorbance imidazole?
Hi Fellows,
could someone please point me towards the source of a known high purity
imidazole
with low absorbance at 280 nm? I am facing the problem of detecting a low
absorption protein
in high imidazole background after IMAC gradient elution. In the UV spectra of
the
2 imidazoles I checked there is some contaminant that absorbs at 280…
Thx, BR
----------------------------------------------------------------------------------------
Bernhard Rupp
Marie Curie Incoming International Fellow
Innsbruck Medical University
Schöpfstrasse 41
A 6020 Innsbruck – Austria
+43 (676) 571-0536
bernhard.r...@i-med.ac.at<mailto:bernhard.r...@i-med.ac.at>
----------------------------------------------------------------------------------------
Dept. of Forensic Crystallography
k.-k. Hofkristallamt
Vista, CA 92084
001 (925) 209-7429
b...@ruppweb.org<mailto:b...@ruppweb.org>
b...@hofkristallamt.org<mailto:b...@hofkristallamt.org>
http://www.ruppweb.org/
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