Dear all,
I recently collected some 10 degree wedges of data on a very small
membrane protein. The data processing so far is not working well. XDS
is the only software that process my data and give a space group and
cell parameters. However, I ended up with a merged dataset with low
completeness at low resolution. My data is not overloaded. This is a
very small protein shot with 5um mini-beam. I checked the FRAME.cbf
file and looks like XDS is not picking the spots at low resolution
correctly. It missed a good portion of my low res spots in every
datasets I checked and picked more at high res, which are weaker. Then
the indexing step will reject most of my spots for "too far away from
ideal position". imosflm was able to pick the spots correctly, but
does not index. I can send the FRAME.cbf file and the imosflm picture
if anyone thinks that will he useful to see. Any suggestion on how to
make XDS, or imosflm, correctly working for me is greatly appreciated.
Thanks a lot!
Best regards,
Fei
Fei LI
Graduate Assistant
310 Biochemistry Building
Department of Biochemistry and Molecular Biology
Michigan State University
East Lansing, MI
48824
