Hi Glenn -
I have nothing systematic, but I remember that transducin-alpha (1TND)
was crystallized at -12 C, with 20% glycerol in the mother liquor. (The
crystals grew at higher temperatures, but weren't as good.) I worked on
this project briefly in grad school, and I remember that looking at the
crystals was a big nuisance - you had to take the trays out of the -12
freezer, run to the cold room, and look at them quickly before they
warmed up too much!
- Matt
On 5/30/13 7:26 AM, Glenn Masson wrote:
Hello CCP4BBers,
I am currently playing with some crystals that seem to enjoy lower
temperatures, and I was thinking of breaking the 0°C threshold.
Looking for examples of this in the literature is problematic, as
searching for examples in the PDB (Under the advanced search-> crystal
properties-> temperature (K)) turns up a large amount of false
positives. Many otherwise supremely intelligent people seem unable or
unwilling to grasp the concept of Kelvin (it's amazing how many
protein structures were solved only 22 degrees above absolute zero...).
I was wondering if anyone has much experience in this area? I see a
few structures e.g. 2Z97 (-5°C) and 4H0W (-2°C), but I was wondering
if anyone has a more systematic knowledge, some more examples, and
what the parameters and best practice of this technique are.
Many thanks,
Glenn Masson
MRC-Laboratory of Molecular Biology
--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
