Dear all, I would be interested in comments/opinions about what is your experience on which would be the best mammalian cell culture vector/cell line/medium combination for transient expression to get the best yields. Depends, of course, but anyway obviously there are differences, HEKs are suppose to be easy to transfect, but which flavour and how and what medium or would you prefer CHO? (probably more so for stable cell lines?)
...etc. vectors...? Or does it make a difference (it seems to). Good reviews/comparisons? is it worth going beyond DMEM and supplements? (what do you add in?) Stupid questions - trying to educate myself a bit here.. and i am willing to spend a bit or at least know what difference it makes (time is money anyway ...and worse). and we are not doing suspension at the moment, but could consider it if it seems like a good idea. Thanks for the tips.., Tommi
