Dear Alex,

i work with Purifier.
To my mind, this is a very nice system. High reliability. We are a lot of differents users and we share the same system, some of us are not very implicated in the cleaning and maintenance procedure, nonetheless the Akta works well. Akta Purifier with 3 wavelenghts is very usefull. For the best usage, you have to use it in cold room, or with a good cold cabinet. I saw one time the Akta Avant (for testing), it looks like a ferrari (shining red color for the exterior shell ;-) ), this product propose you to prepare Buffer with stock solution. Your sample after collection, is keeping in cold compartment. This product have a lot of very usefull and high-tech specs. But i think the good question is : "what i want do with my FPLC" Because, the cost is very different, and if i have to choice, for my usage, i prefer to buy Purifier, with good fractionation system, and some good column instead of the "ferrari". The major interest with the Avant, is that this system has the capacity to work, at high speed (fast flow) like the Akta Express, and the other side, you can set very advanced parameters, to prepare your buffer. Nothing else to my knowledge can prepare the same variety of buffer automatically. And finally, you can develop very fine and advanced purification protocol.

Hope to help you.

Nicolas

Le 28/03/13 20:39, Alexandra Deaconescu a écrit :
Dear crystallography enthusiasts:

We are shopping for a new fplc system. While have pretty much decided to buy a  GE 
Healthcare ("Akta") rather than a Biorad system, we are interested in finding 
out your opinions about the new models that are currently on the market, such as the Akta 
Avant and the Akta Pure, and how they compare to the Purifier.

Any comments would be much appreciated!

Bests,
Alex

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