Hi Tom, some suggestions for you:
You should calculate a selfrotation function and see if you can learn something from it. You should definitely run Matthews Coefficient and see if you get a brilliant idea there And congratulations to your first crystal structure it looks really great, for a starter this is the right resolution to play with and get exposed to all the programs used in the crystallographic community. You got my other email off-the board but for the record keepers here, in case you were offended by my early April fools joke I do apologize. And the second part in the private email still holds true. Jürgen P.S. may I use your data in an X-ray workshop ? On Mar 27, 2013, at 5:26 PM, Alexander Aleshin wrote: But the amount of time spent on turning a protein into a publishable structural data is pretty much same, if not larger. There are no low hanging fruits any more. On Mar 27, 2013, at 11:50 AM, Frank von Delft wrote: Is it too much to dream that Tom has set a trail-blazing precedent and demonstrated to us all how unnecessary it is be anal about our oh-so-precious data and structures that in the year 2013 are almost completely useless without a huge dollop of other experimental data...? On 27/03/2013 18:32, Anastassis Perrakis wrote: I think it will be the first time in 15 years I will disagree with Tim. I personally found the posting of Tom van der Bergh irritatingly disrespectful in many levels. 1. It does not respect my mailbox capacity 2. It does not respect CCP4 developers posting output from phenix.refine 3. It does not respect his supervisors and colleagues who (right now) look like fools (to me) 4. It does not respect himself, as I actually suspect he is a proactive motivated student who came out as a bit of a fool These said, I am rather easily irritated these days, so I will not comment on the irritable character of the email. As for the answers, some were funny, some were informative, some funny and informative. Not too much political correctness please, because we will soon start calling disordered loops positionally challenged polypeptide segments (*). Tassos (*) joke stolen from Thomas Schneider talk @Stanford, 1998. What a great meeting...! Dear so-far-posters, I do not know Tom Van den Bergh, nor do I know his background, nor the history of the data, nor the reasons why he may have sent it to this list (although I think he did it to ask for help), but I find these answers irritatingly disrespectful and nasty. No regards to the ones addressed, Tim ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
