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On 20/03/2013, at 7:59 PM, Harsh Bansia <spideysp...@gmail.com> wrote: > Sorry for a simple and non-CCP4 question. > I have determined the structures of three different mutants of a thermostable > protein by X-ray crystallography method. I feel that Mg2+ has a role in > protein stability. > So I want to perform a thermal denaturation study by CD spectroscopy both in > presence and absence of Mg2+ ion. > In this regards, what should be the suitable buffer for CD studies? May I use > PBS buffer ? Since phosphate sequester divalent cations like Mg2+. Is it > advisable to use PBS buffer. If so, what is maximum concentration of Mg2+ ion > that can be used say e.g. 5 mM? My protein was in Tris buffer and lyophilized > and have theoretical pI =4.56 and maximum activity at pH 8.4. > > Thanking you in advances, > harsh
