Adding 1-10mM copper sulfate is often a good way to oxidize disulfide bonds, although some proteins cannot tolerate this treatment.
Mike ----- Original Message ----- From: "Jacob Keller" <j-kell...@fsm.northwestern.edu> To: CCP4BB@JISCMAIL.AC.UK Sent: Thursday, February 28, 2013 3:09:18 AM GMT -08:00 US/Canada Pacific Subject: Re: [ccp4bb] disulfide engineering Along these lines, what reagents do people use to promote disuflide bonds, i.e., the "anti-DTT?" JPK On Thu, Feb 28, 2013 at 2:06 AM, David Briggs < drdavidcbri...@gmail.com > wrote: You might want to try "Disulfide by design" http://cptweb.cpt.wayne.edu/DbD2/ Cheers Dave On Feb 28, 2013 6:55 AM, "Careina Edgooms" < careinaedgo...@yahoo.com > wrote: Dear CCP4 members I wish to engineer a disulfide bond at the dimer interface of a protein I am working with. Does anyone know of any available software to assist with this? Best Careina -- ******************************************* Jacob Pearson Keller, PhD Postdoctoral Associate HHMI Janelia Farms Research Campus email: j-kell...@northwestern.edu ******************************************* -- Michael C. Thompson Graduate Student Biochemistry & Molecular Biology Division Department of Chemistry & Biochemistry University of California, Los Angeles mi...@chem.ucla.edu
