On Mon, Feb 25, 2013 at 8:02 AM, lei feng <spartanfeng...@hotmail.com> wrote: > I need your suggestion for slowing down crystallization for my protein > my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9), but > it crystallize too fast. In 1 hr I can see tons of tiny needles. > Can anyone give me some suggestion on how to slow down the process? I used > lower conc. of potein, lower conc. of PEG ( 10%), it helped a little bit, > giving me small rod crystal. but no improvement after that.
Sometimes you can do this by adding a tiny amount of glycerol to your protein solution - I've seen 0.5% make the difference between awful plate clusters and nice individual crystals. I think it's also possible to use a combination of low concentrations and micro-seeding, but I've never done this personally. -Nat
