Dear Xianchi, unfortunately, dissociation rate constant kd 10^-6 s^-1 was just beyond the limit of Biacore in 1999 (e.g. see Fig. 1 in http://www.ncbi.nlm.nih.gov/pubmed/10556876). I am not sure about these days. As Juergen noted, you may have a problem with rebinding (you could try to reduce amount of the ligand on the chip, see other tricks here http://users.path.ox.ac.uk/~vdmerwe/internal/spr.pdf). Regarding reviews, David Myszka writes enjoyable reviews on SPR. Hope that helps, Tomas
On Wed, Feb 20, 2013 at 5:03 PM, xianchi dong <dongxian...@gmail.com> wrote: > Dear all, > > Recently I have measure a set of kinetic data of a receptor ligand > interaction using BIAcore 3000. To my surprise, the dissociation rate is > very low (~ 10e-6). During the measurement, I use a long dissociation time > (2 hours) .I repeat several time which give very similar results. So I am > wondering if the BIAcore can measure such a low off-rate kinetic. What is > the limitation of BIAcore? Any review about that? > > Thanks in advance. > > Xianchi Dong > Research Fellow > Children's Hospital Boston > Harvard Medical School Dea
