Jacob, I recall Dorothy Hodgkin or Guy Dodson showing a slide (mid to late 1970s) with insulin crystals having grown in the Islets of Langerhans. As you say, quite remarkable. John
Prof John R Helliwell DSc On 15 Feb 2013, at 19:44, Jacob Keller <j-kell...@fsm.northwestern.edu> wrote: > Dear Crystallographers, > > I was looking at some live, control HEK cells expressing just eGFP, and to my > great surprise, saw littered across the dish what appeared to be small > fluorescent needles (see attached--sorry about the size, but it's only ~1MB > total.) Can these possibly be fortuitous protein crystals? They were too > small to mount I think, and for what it's worth, parallel-transfected HeLa > cells did not have these things. But, some needles could be seen in the DIC > images as well, and the needles were only fluorescent with GFP filter sets, > and not CFP, YFP, or texas red filters. I thought of whale myoglobin > crystallizing on the decks of ships, but never thought I would see this.... > > Jacob > > -- > ******************************************* > Jacob Pearson Keller, PhD > Postdoctoral Associate > HHMI Janelia Farms Research Campus > email: j-kell...@northwestern.edu > ******************************************* > <GFP_crystals_DIC.png> > <GFP_crystals_Fluorescence.png>
