Hello everyone, Thanks for the great interest in the kit, I have at least 30 requests already. For those of you asking about next steps, it will be for me to send a material transfer agreement for you and your office to sign. I will need the contact information, email, phone, University name and address. Once this is complete, I can send the kit.
Due to the overwhelming response, it will be most appreciated if people are willing to pay for the shipping on dry ice. We generally use Fed Ex, but have used UPS as well. Thanks again, Jessica Jessica Silvaggi, Ph.D. Licensing Manager UWM Research Foundation Phone: 414-906-4654 Mobile: 508-769-5171 jsilva...@uwmfdn.org<mailto:jsilva...@uwmfdn.org> 1440 East North Ave. Milwaukee, WI 53202 From: Jessica Silvaggi Sent: Wednesday, February 13, 2013 3:41 PM To: 'CCP4BB@JISCMAIL.AC.UK' Subject: UWM is seeking testers for a new protein expression kit Hi everyone, Dr. M.L.P. Collins at the University of Wisconsin-Milwaukee has developed a new method for making large quantities of active, viable proteins using Rhodospirillum rubrum as a host. R. Rubrum is a bacterium that has traditionally been studied for its simple photosynthetic system, has demonstrated recent success in expressing high-yield protein. To provide a brief overview, R. rubrum possesses the unique characteristic of forming an intracytoplasmic membrane (ICM) in response to membrane protein synthesis. The ICM is non-essential for growth and can incorporate foreign and over-expressed membrane proteins without disrupting normal cellular function. This characteristic has stimulated the expression of active and correctly folded membrane protein where other systems have failed. In an attempt to gather additional testing data, the UWM Research Foundation would like to provide FREE protein expression kits to laboratories or universities that are having difficulties with their current method of producing large-scale proteins. Having limited resources, our researchers have only had the ability to test a number of proteins but thus far have seen exceptional promise. We currently have 8 other universities or companies using the new kit and are now ready to distribute to other participants. The link listed below provides a more detailed explanation of the testing process including the specific proteins currently being tested. You may also view Dr. Collin's publication on her findings of using R. rubrum as a host. If you are interested in learning more about this new system or have any additional comments, you may email me at jsilva...@uwmfdn.org<mailto:jsilva...@uwmfdn.org>. Testing Kit Link Slides http://www.uwmresearchfoundation.org/getdoc/2a36a544-e47c-442e-baaf-9fb3442694f0/Slides-for-Kit-Testing-2013.aspx Dr. Collin's Publication Butzin, N.C; Owen, H.A.; and M.L.P. Collins. 2010. A new system for heterologous expression of membrane proteins: Rhodospirillum rubrum. Protein Expression and Purification, 70: 88-94. http://www4.uwm.edu/letsci/biologicalsciences/facultystaff/collins/upload/Butzin_et_al_2010.pdf
