Am Mittwoch 06 Februar 2013 17:10:35 schrieb Yuri Pompeu: > Dear All, > I am trying to probe the existence of a disulfide bond on the surface of my > protein. I have attempted Ellman´s and my results were not as clear as I > would have hoped for. I am not a sulfur/cysteine chemist and would > appreciate the advice on what experiments to try! Thanks a bunch > YAP >
Hi, You might also try ABDF labeling of free cysteines. Separate the protein on a native gel and look which of your bands show fluorescence under UV. If you compare it to a protein with known free cysteines, you might also get an idea how much closed your disulfide bridge is actually. Christian
