-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Mike,
I'd be surprised if the book was from Hampton: The pH-value of Hampton solutions refers to the stock solution of the buffer before setting up the final solution, and can differ a lot from your drop's pH - e.g. in the presence of Imidazole or other buffering precipitants. I might be wrong but believe that if you prepare the solution yourself, a chemist should be able to explain to how to do the maths yourself. Regards, Tim On 01/17/2013 06:38 AM, Mike John wrote: > > Hello, > > Shameful and sorry for asking this simple question, it looks like > this when first starting a new setup in so-called structural > biology. I remmeber a book of, probably, Hampton, in which there > are tables of pH optimization for many buffers. For example for > buffer TRIS, the table will list how many drops NaOH/HCl needed to > change pH from 7.0 to 7.2, etc. This is useful for crystallization > pH optimization, where can I buy or download this info? > Alternatives? > > Thank you very much! > > Mike > > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQ982/UxlJ7aRr7hoRAldcAKCJCD3q9WGdEMC/UuF7UVKv5k30RgCfR7r1 ajsWl16ZEyXqSASuvIERQWM= =G64g -----END PGP SIGNATURE-----
