Hi all, I was wondering if anyone out there has any anecdotal evidence of intein tag cleavage buffer compatibilities outside those suggested in the IMPACT manual (pH between 8 and 9, 0.1-2M NaCl or 0.1% Triton X-100). Specifically I'm looking for whether I can reduce the pH to <8, acceptable additives like glycerol or xylitol, or crystallization friendly detergents (i.e. not Triton/Tween). This is the Sma VA Intein tag if that helps.
Telling me what doesn't work would be as useful as what does. Any assistance is appreciated. Cheers, Katherine
