El 21/12/12 05:16, dengzq1987 escribió: > Hi all, > recently, i perform an EMSA experiment.when i prepared the DNA with a 5' > poly(G)-tailed ,i find that it formed guanine tetraplexes .how can i > prevent this happen,or has any method to disrupt the > guanine tetraplexes structure? any suggestion is appreciation. > > > dengzq >
Hi, How you know the tail forms tetraplexes? Couldn't be other type of structure? Tetraplexes are stabilized by potassium and, to a lesser extent by sodium cations. Try to avoid them in your buffer or to keep them to a minimum. Or you can use lithium as counterion, it is too small to stabilize the tetraplexes. Another option is to add a complementary poly(C) oligo before running your EMSA. Cheers, -- Miguel Architecture et Fonction des Macromolécules Biologiques (UMR7257) CNRS, Aix-Marseille Université Case 932, 163 Avenue de Luminy, 13288 Marseille cedex 9, France Tel: +33(0) 491 82 55 93 Fax: +33(0) 491 26 67 20 mailto:miguel.ortiz-lombar...@afmb.univ-mrs.fr http://www.afmb.univ-mrs.fr/Miguel-Ortiz-Lombardia
