Dear Roger,
In my humble opinion, the qualitative knowledge that the complex
actually forms (established through pull down assays, gel filtration
etc) is probably far more important than the Kd values in solution. In
any case, the crystallization is done at very high concentrations, far
above the Kd values of the interacting partners. So having a thumb rule
is not necessary. The knowledge of the stoichiometry of the binding
partners may actually be more important to get a more homogenious complex.
In a recent complex I've worked on, we determined the Kd only after
solving the structure.
.
cheers
Ganesh
Le 07/12/12 16:11, Roger Pickman a écrit :
Dear all - is there a rule of thumb for favourable values of Kd, kon
and koff of protein-protein or protein-dna complexes for protein
crystallisation? Are these measurements useful in crystallisation, or
should one just put it down a gel filtration column, hope for a
complex and not worry? If anyone can point toward a reference, i'd
appreciate it.
Roger
