Suggestion: What does your plunge of a loopful of the buffer (no crystal) into liquid nitrogen tell you?
________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Yuri Pompeu [yuri.pom...@ufl.edu] Sent: Friday, November 30, 2012 7:22 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] To cryo or not to cryo... Dear community, I have what seems to be a pretty decent single crystal that grew from a screen set up 2 weeks ago. I am trying to reproduce it but so far I have not succeeded. I am however afraid the crystal that did form will start to deteriorate. So this brings me to dilemma, I feel like I should try and mount this crystal and shoot it. But since I only have 1 sample, I do not want to mess this up... I am inclined to try cryo conditions, but I am afraid the addition of a cryo such as glycerol could destroy the little guy. The crystal formed in 30% PEG 4000, 0.1M NaCitrate pH5.6 and 0.2M NH4AcO, I wonder if this is a cryo condition already? Any suggestions would be appreciated. best,
