Hi, Dear group, I recently collected a dataset about 2.5 A and integrated with P4. When I tried phaser I got a sol file looks like this, is this real solution? Is LLG high enough?
SOLU SET RFZ=5.2 TFZ=10.3 PAK=0 LLG=239 LLG=366 TFZ==20.9 SOLU SPAC P 41 SOLU 6DIM ENSE ensemble1 EULER 50.265 0.217 219.800 FRAC 0.50029 0.49916 -0.00408 BFAC -0.04000 SOLU ENSE ensemble1 VRMS 0.639 SOLU SET RFZ=5.2 TFZ=10.0 PAK=0 LLG=239 TFZ==11.2 LLG=365 SOLU SPAC P 41 SOLU 6DIM ENSE ensemble1 EULER 128.607 179.813 38.677 FRAC 0.49991 0.49905 -0.00208 BFAC -0.06264 SOLU ENSE ensemble1 VRMS 0.642 Looks like there are two solutions,but in the output pdb file, I only see one solution (homo-tetramer). So I took this output pdb and run first round of refinement with Refmac, somehow, the structure fall apart if I use restraint refinement( lots of atoms are not connected any more ). If I use rigid body refinement, it's ok and Rfree is 52%. Does that mean the solution might not be correct? What's the acceptable Rfree for the initial refinement? Thanks a lot. Rui
