Thanks for the confirmation. Raji
On Thu, Oct 25, 2012 at 10:44 AM, Rob Gillespie <robert.gilles...@duke.edu>wrote: > Put me down at another person who re-suspends bacterial cell pellets in > 4-5 volumes of buffer. > > > > > On Thu, Oct 25, 2012 at 9:15 AM, Opher Gileadi <opher.gile...@sgc.ox.ac.uk > > wrote: > >> It makes sense to use a fixed ratio of resuspension buffer to cell >> weight; we weigh the pellets after centrifugation, then suspend in at least >> 4-5 volumes (ml/gr) of buffer. >> > > -- Raji Edayathumangalam Instructor in Neurology, Harvard Medical School Research Associate, Brigham and Women's Hospital Visiting Research Scholar, Brandeis University
