-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Rhys Grinter,
when you deposit a model, I think it is the consens to believe that you as its author have done your (reasonably) best to create that model given the data available, i.e. if you come to the conclusion that you cannot justify building a loop, that's fair enough. There a model building tools like arp/warp and probably also buccaneer, and I assume they take e.g. Ramachandran geometry into account. Give them a try and see if what these achieve! Best, Tim On 10/17/2012 06:05 PM, RHYS GRINTER wrote: > Hi All, > > This is a fairly basic question coming from a novice > crystallographer, but I was wonder at what point it's reasonable to > say 'I can't model that loop!'. I'm currently working on a couple > of structures and there's density for where the a flexible loop on > both structures goes, but there isn't good density for side chains > (even aromatic ones) and even if the loop can be closed it doesn't > seem like the Ramachandran plot will ever be happy. Both data sets > are around 2A so it's not a question of poor density overall > > I was wondering if there was a consensus for when density is > unmodelable, or if there are any tips for fitting residues into > poor density. > > Thanks in advance, > > Rhys Grinter PhD Candidate University of Glasgow - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQfuMYUxlJ7aRr7hoRAl6PAKD7vHV3y43wYBcukxMjrAmXBj7k2gCgj3US PqGN6uzMRwk1563MTn4sRQQ= =lp9r -----END PGP SIGNATURE-----
