Hi, Thanks for answering my question. I think I'd better provide more informations. These four images were taken from one crystals. The distance of image1-2 is 250mm, and image3-4 is 100mm. I checked more than 10 crystals and results were similar. The spots look very large. The lowest resolution is about 6A. As my protein can hydrolyze ATP, so protein buffer with 5mM of ATP. There is 0.2M of MgCl2 in precipitant buffer with PH7.0. 0.06M of CsCl can improve quality of crystals. I also setup control, in which target protein was not added, and could get nothing in it. I don't think it is a protein crystal. But salt spot should not be so large. And the rings are not just ice-ring. As I got crystal first in hampton crystal screen kit with MgCl2, TrisHCl and PEG4,000, there are only rings in images from 5-6A to about 3A.
2012/10/12 THOMPSON Andrew <andrew.thomp...@synchrotron-soleil.fr>: > Hi Chang > No mention of the resolution limit / oscillation range (I think I can see an > ice ring, so I would guess 2.5 A?), but it looks like salt to me, with some > weaker satellite peaks that may be something weird like an incommensurate > phase. > Did you try to index? > Cheers > Andy > ________________________________________ > De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Chang Qing > [robie0...@gmail.com] > Date d'envoi : vendredi 12 octobre 2012 08:11 > À : CCP4BB@JISCMAIL.AC.UK > Objet : [ccp4bb] Strange diffraction image > > Hi, everyone: > > I just got some strange diffraction images from crystals with > triangular pyramid shape. I think this should not be protein > diffraction. I never saw so strange images like this. Does anyone know > what it is? Is it a kind of salt diffraction? > Thank you very much > > Chang
