Hi all, I recently got some hits from a Mosquito grown at 18C with PEG4000 and 10% propanol. I've tried reproducing these hits using the standard 24 well format with no success, even with playing with PEG and propanol concentrations. The initial screening solution was from a kit that's been sitting in the cold room for the better part of 2 years. Does anyone here have any tips or tricks in reproducing crystals grown from organic solvents?
Many thanks in advance, Peter