Dear Crystallographers: #off topic
I am trying to calibrate some microscopic imaging intracellular pH measurements, but the usual nigericin (small pore-forming K+/H+ exchanger) technique does not seem to be working well. Maybe it actually never works well? Anyway, I was think of trying to use something which forms larger pores to allow all things to equilibrate quickly, but not allow the bigger pH-sensitive dye (SNARF) to escape? I don't care, by the way, if it kills the cells, as the calibration is done it the end, provided that the cells don't float away off the coverslip. Does anyone know of a candidate for such? JPK -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program email: j-kell...@northwestern.edu *******************************************
