On 09/06/2012 07:48 PM, Qing Luan wrote:
I built a molecular replacement model
What is the model based on (i.e. how much sequence identity you have)? Did you try something other than CNS (specifically for twinning detection)? Did you check the patterson map and/or self-rotation for off-origin peaks?
36% solvent is highly unlikely given the resolution. Did you try molecular replacement with individual domains? etc, etc, etc
