Dear all I made deletion mutation of a stretch of 20 amino acids on my protein. I can purify and crystallize wild type protein but not the mutated. Mass spec on gel separated protein shows degradation of mutant losing about another 150 amino acids. Is there any way of purifying this non-stable protein? I know nature has designed proteins to be stable.
All steps are done at 4 C and protease inhibitor added during cell lysis for both proteins. Thank you.
