Hi Faisal,
The solutions from BALBES have already gone through multiple rounds of refinement which minimize the R/Rfree. If you make a number of drastic changes to side chains or add waters, the R/Rfree should go down. However if the structure fits the density well (with no side chains needing major adjustment) and you have not yet added waters... you will not notice the R/Rfree go down. What are you R/Rfree values? ------------------------------------------------------------------------------- Greg Costakes PhD Candidate Department of Structural Biology Purdue University Hockmeyer Hall, Room 320 240 S. Martin Jischke Drive, West Lafayette, IN 47907 -------------------------------------------------------------------------------- ** Hard work often pays of in time, but Procrastination always pays off now ** ----- Original Message ----- From: "Faisal Tarique" <faisaltari...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, August 7, 2012 4:40:38 PM Subject: [ccp4bb] balbes solution Dear all i submitted one job in BALBES at YSBL server. The final outcome are showing the result to be definite solution by stating it to be a 99%solution. but when i am refining with refmac, Rwork and Rfree is not coming down despite my several tries. In COOT i can see tye missing density for loop region. the data is of 2.5 angstrom resolution. -- Regards Faisal School of Life Sciences JNU
