Dear ccp4bb,
I am working on refinement of an enzyme structure. I notice an alternative conformation (gauche+ and gauche-) on both residues Phe and Tyr. Here is the image of electron density before refinement. Based on the different map intensity, I am quite sure there is an alternative conformation on both positions. http://i50.tinypic.com/29zaoap.jpg However, after running occupancy refinement by Phenix, I don't see the density map at the gauche- conformation of Tyr residue. Please take a look at the image 2. http://i50.tinypic.com/anzfrn.png So do you think the gauche- conformation of Tyr exists in this structure. Should I keep it in the final model because there is no positive map there? Thank you very much for your input. -- * Ngo Duc Tri * PhD Student Structural Biology Lab School of Medicine - Sungkyunkwan University - Korea Phone: 031-299-6150 - Cell: 010-7774-8210
