Hi Zhao,
I would try a crystal that has not been treated with any dye. Small molecules, particularly drug like compounds, crystallise out in soaking experiments and can give diffraction similar to yours. My worry is that you have non-diffracting protein crystals coated in some small molecule crystals....the question then is whether the addition of the dye has destroyed your diffraction. Try in-situ or a capillary mount to be more sure. A crush test might also tell you someting. Dave David Hargreaves Associate Principal Scientist _____________________________________________________________________ AstraZeneca DECS, CP&SS Mereside, 50F49, Alderley Park, Cheshire, SK10 4TF Tel +44 (0)01625 518521 Fax +44 (0) 1625 232693 David.Hargreaves @astrazeneca.com <mailto:name.surn...@astrazeneca.com> Please consider the environment before printing this e-mail From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of gengxiang zhao Sent: 23 July 2012 21:53 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Question about weird diffraction map Dear CCP4BB, I have a question about my current diffraction map for one of crystals which can be found in the attachment. Basically, the crystals were dyed to initially testify that it belongs to protein. But from this diffraction, even in the low diffract angle, no diffraction spots there----meaning-----this is not a protein crystal? Any experienced idea/questions welcomed to discuss here. Zhao, -------------------------------------------------------------------------- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 2 Kingdom Street, London, W2 6BD. Confidentiality Notice: This message is private and may contain confidential, proprietary and legally privileged information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorised use or disclosure of the contents of this message is not permitted and may be unlawful. Disclaimer: Email messages may be subject to delays, interception, non-delivery and unauthorised alterations. Therefore, information expressed in this message is not given or endorsed by AstraZeneca UK Limited unless otherwise notified by an authorised representative independent of this message. No contractual relationship is created by this message by any person unless specifically indicated by agreement in writing other than email. Monitoring: AstraZeneca UK Limited may monitor email traffic data and content for the purposes of the prevention and detection of crime, ensuring the security of our computer systems and checking Compliance with our Code of Conduct and Policies.
