Hi Leonard, I would treat this like a MS/MS ion search experiment looking for the peptide via MASCOT or similar. Talk to some local Mass Spec people, I'm certain they could identify it and then you'd be closer to an explanation.
John. Dr. John Hinks Syngenta Jealott's Hill International Research Centre Bracknell Berkshire RG42 6EY United Kingdom john.hi...@syngenta.com www.syngenta.com -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Leonard Thomas Sent: 18 July 2012 23:12 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] peptide fragment Hello All, I have been working on a structure for one of the research groups here and have come across a peptide fragment of about 10 residues. The protein forms a tetramer and the peptide is found between two of the monomers. The protein itself does not require a peptide for function or formation for that matter. Has anyone come across similar things? and have a reference. We are trying to figure out where it may have come from. My best guess so far is from somewhere in the purification though I have no idea how long the protein sat around before crystallization. Cheers, Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry Stephenson Life Sciences Research Center 101 Stephenson Parkway Norman, OK 73019-5251 lmtho...@ou.edu http://barlywine.chem.ou.edu Office: (405)325-1126 Lab: (405)325-7571 This message may contain confidential information. If you are not the designated recipient, please notify the sender immediately, and delete the original and any copies. Any use of the message by you is prohibited.
