Dear Amr,
Every protein is different, so one cannot make a general remark.
However, in my experience disulfide bonds are normally not affected by
mercaptoethanol or DTT. I have had one protein where the disulfide bonds
were not made, likely because the protein was produced in E.coli.
However, the protein still crystallized.
Good luck!
Herman
________________________________
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Behalf Of amro selem
Sent: Wednesday, July 11, 2012 10:51 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] sulphur bridge
Hallo every one,
i am working on one enzyme who has sulphur bridge , is using 2
mercaptoethnol or DTT during handling this protein for crystallography
is not desirable .
best regards
Amr
