Hi Min, I agree with Christian Roth, try malonate at high concentration, 2.8 molar is enough. I suggest to adjust the pH to about the value of your xtallization condition. To avoyd salt xtals formation, humidify the environment, some wet paper around the drop can do the job!
cheers anna 2012/7/10 m zhang <mzhang...@hotmail.com> > regaentDear All, > > I am sure this question was discussed before. But I am wondering if anyone > got the same experience as I do. > I got a crystal out of condition with 1M KCl, 1.4M Ammonium sulfate at > pH7. I tried to use glycerol, ethylene glycol, 25% sucrose, paraton-N oil, > or ammonium sulfate itself: The problem is that all the cryo plus original > reagents in the reservoir precipitate the salts out. And more serious > problem is because of high salt in the condition, while I am trying to loop > the crystal, both the drop and cryoprotectant drop form salt crystals (not > sure it is KCl or ammonia sulfate) significantly and very quickly, that > cause my crystal dissolved. My crystal doesn't seem to survive paraton-N > oil. Does anyone here have similiar case? any suggestion will be > appreciated. > > Thanks, > Min >
