Dear all,
I have solved the structure of a protein with twofold NCS. On the axis
relating the protein molecules sits a peptide ligand in two (mutually
exclusive, partially occupied) conformations, effectively interacting
with either protein molecule half of the time. Sorting out the side
chain conformations of the ligand proves difficult.
I would like to impose twofold NCS on the two conformations of the
ligand. In Refmac, I can relate residue n of chain A to residue n of
chain B. Can I also relate residue An of chain C to residue Bn of chain
C? Or is there some other clever way of dealing with this situation?
Thanks.
Andreas
--
Andreas Förster, Research Associate
Paul Freemont & Xiaodong Zhang Labs
Department of Biochemistry, Imperial College London
http://www.msf.bio.ic.ac.uk
