Native PAGE (i.e. BN-PAGE), light scattering (i.e. MALLS) Not quick and easy but could work: AUC (i.e. a sedimentation equilibrium experiment)
-Brad On Tue, Jun 26, 2012 at 9:01 AM, Careina Edgooms <careinaedgo...@yahoo.com>wrote: > Dear ccp4 bulletin board > > I apologise for off topic question. I wonder if anybody knows of a good > method to detect oligomerisation? > I suspect an equilibrium intermediate is forming oligomers based on > tryptophan fluorescence showing an exposed tryptophan becoming buried in a > hydrophobic region. I would like to perform some experiments to prove this. > Cross linking is not working for me and neither is SEC HPLC due to > technical issues with the urea I think. > Are there any other quick and easy techniques that any of you can think of > off the cuff? Shifts in equilibrium unfolding curves done at different > concentrations will probably show something because of the law of mass > action but this is a big job needing many replicates so I wonder if there > are any other simple methods available? > > Thanks > Careina >
