Try adding DNA then dialyzing to low salt (in some microdialyer). ===================================== Phoebe A. Rice Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 http://www.rsc.org/shop/books/2008/9780854042722.asp
---- Original message ---- >Date: Mon, 18 Jun 2012 15:22:46 +0530 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of Arpit Mishra ><ar...@igib.in>) >Subject: [ccp4bb] Fwd: Delivery Status Notification (Failure) >To: CCP4BB@JISCMAIL.AC.UK > > Dear CCP4BB members > > Sorry for off topic, i had question related to > dispersity of proteins of my > interest, i am working on DNA binding protein, > having PI around 9 to > 10,Initially protein was remaining stable at high > salt, but was not able to > concentrate more than 1mg/ml, after using 50mm Arg, > 50mm Glutamate i > overcome the solubility and concentrating problem > but problem now is that > my protein is remaining polydisperse, so i want to > try different > additives, i had tried some detergents and > chaotropic agent,pH ranges.but > it didnt help, so any suggestions to bring my > protein in monodisperse state > that will be really helpful...thanks for your > suggestions in advance > > -- > Arpit Mishra > >
