Hi Qixu Cai,

first off -- as a reminder, there is Phenix mailing list to post
Phenix-related questions:
http://www.phenix-online.org/

Your first question: yes, give it one sequence file containing all.
Your second question: see here
http://phenix-online.org/documentation/autobuild_gui.htm

Pavel

On Wed, Jun 13, 2012 at 5:14 AM, Qixu Cai <caiq...@gmail.com> wrote:

> Dear all,
>
> Sorry for the non-CCP4 question.
>
> My crystal is a complex of two proteins. I use Se-Met SAD method to solve
> the structure, and only one of the proteins is Se-Met protein.
>
> When I use phenix.autobuild to auto-build the model, how do I input the
> sequences? in a file or two files?
>
> And is that necessary to specify the location of MSE?
>
> Thanks a lot for your help.
>
> Qixu Cai
>

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