Hi Jun, In this following article an metallic adapter was used to mount a 192 well sitting drop plate on a conventional thermocycler. http://journals.iucr.org/d/issues/2006/05/00/av5047/av5047bdy.html. However I guess there would be significant evaporation and condensation if you use vapor diffusion setup and cycle the temperatures.
On the other hand, if you do microbatch under oil, then you should be able to easily setup the experiment on a 96-tube PCR plate and mount it on a PCR machine. I guess the temperature can be more precisely controlled this way with no special equipment needed. Especially, the evaporation and condensation during thermo cycles would probably be less problematic. Zhijie From: Jun Ma Sent: Tuesday, June 12, 2012 11:15 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Where to get a thermo-cycling controlled chamber Hi everybody, My experiment need me to try a thermo-cycling method for protein crystallization. So I need a kind of temperature chamber, which can changes temperature quickly, precisely and steadily. Furthermore, it should be relatively quiet when running, because I'm afraid vibration will affect the crystallization of my protein. I just need a small one, which can control temperature from 0 C to 50 C. I'm looking for this for some time, and still don't know where to get it. If anyone can provide information about where to purchase, or how to solve the thermo-cycling control if there's no commercial merchandise, I will appreciate that very much. Any other suggestions are welcome. Jun Ma 2012-06-12
