Hi Jose, >Has anyone worked with, or seen an example of, a solvent accessible disulfide >that cannot be reduced with TCEP?
Something to keep in mind is that TCEP is not very stable with phosphate buffers near neutral pH. >Alternatively solvent accessible disulfides that resist reduction with DTT but >can be reduced with TCEP? This could be due to the effective pH of TCEP HCl (~1.5-8.5) being wider than DTT ~7. Take Care, Sean Seaver P212121 http://store.p212121.com/
