While a comparison of the B factors is easy to do, interpreting its meaning is not. The B factors are affected not only by the motion of the atoms, but by many other factors, including the overall quality of the order of the crystal. The B factors for a model based on a low resolution data set will be larger than those from a model of the exact same protein based on a high resolution data set.
If you wish to derive some sort of structural interpretation to the B factors of your region of interest you have to somehow normalize them relative to the average B factor of the better ordered parts, or, say, the Wilson B. Dale Tronrud On 05/11/12 02:32, <World light> wrote: > Hi, > > I would like to make the comparative study of different mutant structures. > There > is a region which is flexible and missing in some cases. Is there any direct > method of calculating the BAVERAGE of that region. I used the baverage program > in ccp4 for whole structure but would like to see the values with and without > the region. > > Thanx, > > Bishwa
