Hello Ruby,
I would start by verifying the functional status and
monodispersity of your protein. If it appears that you have a good
protein sample, I suggest screening with a more concentrated protein
solution. What protein concentrations have you been you working with?
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
h...@hawaii.edu
On Fri, May 4, 2012 at 1:00 PM, CCP4BB automatic digest system
<lists...@jiscmail.ac.uk> wrote:
> Date: Fri, 4 May 2012 12:39:42 +0530
> From: ruby singh <singh.rub...@gmail.com>
> Subject: <No subject given>
>
> Dear all,
>
> Im a PhD student who started working on Protein crystallization. Its been
> years im trying to get any crystal of that protein. I have tried all
> crystallization screens available.
> but no results. My protein is highy thermostable(till 80C) and pH stable(1
> to 13)...plz if anyone is having any idea or trick let me knw.
