Hi all,
I've been using a Belle technologies anaerobic glovebox with in built
microscope and liquid nitrogen dewar port in an attempt to harvest and
cryo-cool crystals in an oxygen free environment.
I've been having some problems with icing and think this is most likely
due to slow speed that it is possible to plunge loops into the liquid
nitrogen. I've been plunging loops straight into vials preloaded into
an ESRF puck (with pusher). This requires more care than what I would
ordinarily do (i.e. plunging into bulk liquid nitrogen then and
manouvering into a vial afterwards and then clipping said vial into a
cane) and so takes longer - perhaps leading to icing. The latter
technique is not available as the glovebox set up means that the dewar
can only be reached by my right hand - the microscope is in the way!
Does anyone have any experience of a similar set up and if so have some
advice as to how I can overcome this problem?
Many thanks,
Dave Gallagher
--
*David Gallagher *
PhD Student
MRC Mitochondrial Biology Unit
Wellcome Trust / MRC Building
Hills Road
Cambridge
CB2 0XY
Email: dt...@mrc-mbu.cam.ac.uk <mailto:dt...@mrc-mbu.cam.ac.uk>
Phone: 01223 252913
