Dear all, I got a weird solution from Phaser. The background is that, space group C2221, resolution ~4A, in complex with a peptide, and having a apo form structure as the search model. Phaser gave two rotation function peaks with Z > 7. But when searching translation function peaks, Phaser gave many high Z score peaks listed below rather than a single solution. These peaks share same fraction Y&Z but with different X. Most of them pasted the packing validation. The when searching the second copy, each solutions have a single translation peak that showed very high Z score (> 20). I check some of these solutions in Coot, and found that the two copies of each solution has the same relative orientation and each solution shifts several angerstroms in X axis. I also tried C222 and did not get better result than C2221. Any comments or suggestion? Thanks a lot!
Best, Zhiyi # (#) Frac X Frac Y Frac Z LLG Z-score Split #Group raw/top 1 1 0.155 0.436 0.287 +219.80 11.17 0 1 272.58/272.58 2 2 0.350 0.436 0.287 +211.92 10.53 24 1 267.42/267.42 3 3 0.542 0.436 0.287 +211.31 10.48 44 1 266.05/266.05 4 5 0.579 0.436 0.287 +209.66 10.34 40 2 260.14/260.14 5 12 0.267 0.436 0.287 +209.11 10.30 14 2 256.53/256.53 6 4 0.224 0.435 0.288 +208.41 10.24 8 2 261.39/261.39 7 11 0.485 0.436 0.287 +208.28 10.23 40 2 256.95/257.13 8 7 0.191 0.435 0.287 +205.18 9.97 4 2 258.95/258.95 9 15 0.400 0.436 0.287 +201.90 9.70 30 2 254.20/254.20 10 9 0.297 0.437 0.287 +201.79 9.69 17 1 257.75/257.75 11 16 0.449 0.436 0.287 +198.77 9.45 36 1 252.80/252.80 12 17 0.129 0.437 0.287 +195.66 9.19 3 1 252.41/252.41 13 20 0.377 0.436 0.287 +194.95 9.13 27 1 246.37/246.37 14 21 0.422 0.436 0.287 +190.72 8.78 33 1 245.42/245.42 15 19 0.521 0.437 0.287 +190.22 8.74 45 1 246.46/246.46
