Re: [ccp4bb] weird--No protein expression using pET30a

[Yuri Pompeu]

Hello,
Probably a stupid comment on my part, but anyways, make sure your strain has a 
T7 polymerase! (I have forgotten before).
And I agree with the last idea that sometimes it is worth to just start from 
scratch. New construct new vector. It may simply just work.
HTH,
Yuri