James, For a detailed analysis of Matthews coefficients (Matthews probabilities), you can use this web-based tool:
http://www.ruppweb.org/Mattprob/ There is a pull-down menu to select the sample type, and one choice is "protein-DNA complex." Perhaps someone can correct me if I'm wrong, but I can't imagine the results would be very different for protein-DNA vs. protein-RNA. Also, if you already have a crystallographic model, as you say you do, then you know how many molecules are in the ASU and you could calculate it by hand : Vm = (volume of unit cell)/(molecular weight of all unit cell contents) Good luck, Mike ----- Original Message ----- From: "Tim Gruene" <t...@shelx.uni-ac.gwdg.de> To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, March 12, 2012 11:07:29 AM GMT -08:00 US/Canada Pacific Subject: Re: [ccp4bb] Matthews coeff. from model -----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear James, I do not know such a tool, but you can use 140A^3/a.a. and 380A^3/base to calculate the solvent content by hand. Regards, Tim On 03/12/2012 06:35 PM, james09 pruza wrote: > Dear CCP4bbers, >> >> Is there any tool to calculate the Matthews coefficient from a >> crystallographic model of RNA-protein complex? >> >> Thanking you. >> James. >> > - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.11 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFPXjthUxlJ7aRr7hoRAqPSAJ0Zr7H/Zt0w8TnaJvHsc5g5mZbZngCcCTEC inwbgapeZ+O0jfc20pMVS/M= =0cVz -----END PGP SIGNATURE----- -- Michael C. Thompson Graduate Student Biochemistry & Molecular Biology Division Department of Chemistry & Biochemistry University of California, Los Angeles mi...@chem.ucla.edu
