Dear all, I hope you can help me! I solved a structure at 3.0 A res and everything is quite satisfactory, my only concern is the discrepancy between the B factor from Wilson plot (58.8 A^2) and the average B (36.9). It seems strange, usually the opposite occurs... What does it mean? Which may be the reason? What can I check? Some details: Rmerge: 15.0 (51.8) I/σ(I): 7.30 (1.84) 5 monomers/AU R/Rfree: 19.7/23.3 ...ask for other infoes if you need.
Any advice/suggestion is welcome!!
