hi did u have other crystals grew in different conditions in your additive screen ? in my case, i did the additve screen for the condition i got twinned crystals i got crystals grew in few conditions one of them is salt but it still gave me twinned crystals then i tried NDSB-221 and the crystals turned out non-twin
hope it helps by the way, even though its twinned crystal should be ok to solve the structure Mary ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Francis E Reyes [francis.re...@colorado.edu] Sent: Thursday, February 23, 2012 4:06 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] effects of salt on twinned crystals I read your description of the crystals and Figure 4 of the following paper came to mind. Post-crystallization treatment in lower PEG eventually allowed them to tease the bundles apart. 1. MacRae, I. J. & Doudna, J. A. An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer. urn:issn:0907-4449 63, 993–999 (2007). F On Feb 22, 2012, at 10:59 AM, Peter Hsu wrote: > Forgot to mention, that this 2.5-3A diffracting crystal was the same one that > I have been unable to index and suspect are twinned due to the presence of > these other fused crystals in the same drop. > > Thanks for any input. --------------------------------------------- Francis E. Reyes M.Sc. 215 UCB University of Colorado at Boulder
