Dear all; I have a structure at 3.3A resolution of about 140kDa protein containing eight domains, in tetragonal space group.I also have the structure of most of the individual domains.I almost refined the structure in all the possible space groups, the best space group at the moment are the P42 with the R/Rfree of 32 and 38%. Could somebody suggest what else I should try to to get better R/Rfree values. I am using Phenix for refinment. Regarding the protein it contain quite flexible domains.
Thanks in advance for your suggestions. Regards, Bashir -- Muhammad Bashir Khan ************************************************** Department for Structural and Computational Biology Max F. Perutz Laboratories University of Vienna Campus Vienna Biocenter 5 A-1030 Vienna Austria Austria Phone: +43(1)427752224 Fax: +43(1)42779522
